CSPD and blocking your blots

[Dave B]

I'm using CSPD-plus with my BRL Photogene kit for nonrad detection on
Southern blots. The kit normally uses Lumiphos 530 but I ran out. CSPD is
also a substrate for SA-AP conjugate and so should work in place of
Lumiphos 530. I went through the procedure as normal and I saw my bands
but there was also high background. Any idea how I can correct this? 

The normal blocking solution is 3 percent BSA in TBS-tween for 1 hr at 65
degrees.   The Southern Light Kit from Tropix (where I got the CSPD comes
with something called I-block). In the original Photogene Kit protocol
there is a final 1 hr wash with their Final Wash Buffer but I don't know
what is in it except there is some formamide. 
Any ideas will be greatly appreciated.

Dave B.
dboyd at cc.umanitoba.ca

-- 
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