PCR with Pwo DNA polymerase from Boehringer
klaus.lun at urz.uni-heidelberg.de
Fri Dec 13 09:05:25 EST 1996
Don J. Katcoff wrote:
> We have been trying to use Pwo DNA polymerase from Boehringer for PCR,
> but have been having difficulties. Using the same template and primers
> but other taq polymerases we have no problem. We are interested in
> particularly high fidelity. Any help would be appreciated.
> Don Katcoff
> katcoff at brosh.cc.biu.ac.il
Increase the extension time of your reaction (2.5 min per kb amplified).
This really helps, I had the same promlem too, a reaction that worked
with Taq Pol. didnt work with Pwo or Pfu. I just increasd the extesion
time, and ... it worked ..
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