denaturing DNA in 0.12Mpb

John Brennand john.brennand at gbapr.zeneca.com
Fri Dec 13 07:56:01 EST 1996


boiling in anything is certain to give 100% denaturation ! - the problem
is re-annealing upon cooling 

to reduce this effect try: lower salt, increased volume (ie more dilute
DNA sample), snap freezing the sample.

it really depends on what you want to do with it after boiling 

john



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