Sequencing an A-T region

Tom Robinson twrobinson at ucdavis.edu
Fri Dec 13 13:31:41 EST 1996


Have an autmated ABI 377 and have been try to sequence an ITS region
that is 80% A-T. this is a 600bp pcr fragment. Cant tell if the initial
amplification is a stutter problem but seq. is garbage. Would cloning
help and how would i know which clone would be the correct sequence? So
far have done everything possible on the seq. end of things and did all
the basic optomizations for pcr.



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