Sequencing an A-T region

[Tom Robinson]

Have an autmated ABI 377 and have been try to sequence an ITS region
that is 80% A-T. this is a 600bp pcr fragment. Cant tell if the initial
amplification is a stutter problem but seq. is garbage. Would cloning
help and how would i know which clone would be the correct sequence? So
far have done everything possible on the seq. end of things and did all
the basic optomizations for pcr.