Dr Alok Adholaya
aloka at teri.ernet.in
Fri Dec 13 04:38:37 EST 1996
I would like to know if there are any ways of conforming the
differentially expressed clones isolated using Differentail display
PCR (DDRT-PCR) other than the standard Northern blot. I am using a
system where the availability of total RNA is a premium ( Not to
talk of Poly A RNA!!!). I am looking for ways and means to check
for the differential expressed nature of the clones where i would
not require large quantities of RNA.
Could anyone suggest me the refrences where RNASE Protection Assay
( RPA) has been used for conformation in leiu of Northern ?
P.S : I want to know the opinion of those of you who are
using/used Silver staining DNA sequencing system of Promega Corp
? Is it as sensitive and easy as made out to be ? Does it "really"
replace the radioactive mess which we exopse ourself when we do
\\ // \\ // \\ // \\
Dr Alok Adholeya \\': | \\ // | :,\\': | \\
Fellow \ | | \\ // | | // \\ | | \\
Microbial Biotechnology \\ | :,\\': | // \\ | :,\\
TATA Energy Res Inst,
Habitat Pl, Lodi Rd, New Delhi, 110003 India
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