in-gel kinase assay

Glenn Jenkins gjenkins at facstaff.wisc.edu
Sat Dec 14 15:00:51 EST 1996


Yea I have some advice -  don't use it. This assay is not very sensitive,
has a major potential for artifacts (ie to kinases running at similar
mobilities and phosphorylate the same substrate) and it is an expensive
assay that requires large amounts of P32-ATP.

This is just my 2 cents.


Glenn Jenkins


In article <58s24u$j6s at news.cuny.edu>, jiang at genectr.hunter.cuny.edu wrote:

> Hi: if anybody has hands on experince on in-gel kinase assay? Please
> gave some advice. Thanks a lot.
> Fan

-- 
Glenn H. Jenkins
Dept of Human Oncology
University of Wisconsin
Madison, WI  53792

Ph (608) 263-4084
Fx (608) 263-4226



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