in-gel kinase assay
gjenkins at facstaff.wisc.edu
Sat Dec 14 15:00:51 EST 1996
Yea I have some advice - don't use it. This assay is not very sensitive,
has a major potential for artifacts (ie to kinases running at similar
mobilities and phosphorylate the same substrate) and it is an expensive
assay that requires large amounts of P32-ATP.
This is just my 2 cents.
In article <58s24u$j6s at news.cuny.edu>, jiang at genectr.hunter.cuny.edu wrote:
> Hi: if anybody has hands on experince on in-gel kinase assay? Please
> gave some advice. Thanks a lot.
Glenn H. Jenkins
Dept of Human Oncology
University of Wisconsin
Madison, WI 53792
Ph (608) 263-4084
Fx (608) 263-4226
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