GST-fusion protein is insoluble.

[lina at PUBMS.PKU.EDU.CN]

Dear Netters:
  I am trying to express a gene from rice in E. coli, using the GST-fusion
protein system, but perhaps due to its high Cys content (17 Cys out of a
total of 131 a.a.), the expressed product is insoluble. How should I
solublize and renature it without the loss of its affinity to the
glutathione-agarose? 
  Thanks for any help in advance.




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          Li Na
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          Peking University, Beijing, 100871
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