GST-fusion protein is insoluble.

lina at PUBMS.PKU.EDU.CN lina at PUBMS.PKU.EDU.CN
Mon Dec 16 02:08:08 EST 1996


Dear Netters:
  I am trying to express a gene from rice in E. coli, using the GST-fusion
protein system, but perhaps due to its high Cys content (17 Cys out of a
total of 131 a.a.), the expressed product is insoluble. How should I
solublize and renature it without the loss of its affinity to the
glutathione-agarose? 
  Thanks for any help in advance.




          ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
          Li Na
          Bldg 46, Room 1033
          Peking University, Beijing, 100871
          P.R.China
          Tel. (010)62754249 (O)
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