Library Screening

[Rajesh Kumar]

Hi Netters,

I am screening a genomic library made up in lambda ZapII vector. I want to
know the growth conditions so as to get 20 000 plaques per 150mm plate.
Right now I am following Maniatis (NZY plates, plaques are grown for 12h
at 37C), but I do not get more than 2 000 plaques (almost touching each
other) per plate. The size of my plaques is almost equal to a well grown
bacterial colony.
Any suggestions in this regard will be appreciated. 

Thanks.
Rajesh 
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Rajesh Kumar, Ph.D.				Phone : (804) 982 1662
Department of Cell Biology, Box 439		Fax   : (804) 982 3912	
University of Virginia, School of Medicine
Charlottesville,Virginia 22908, USA       http://www.med.virginia.edu/~rk6n
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