Differential Display: best kit/primers?
Paul A Bucciaglia
bucci001 at maroon.tc.umn.edu
Tue Dec 17 15:12:56 EST 1996
I'm getting ready to try DDRT-PCR and would like to get comments on good
or bad experiences with various kits. It seems like the kits are a good
deal based on the cost to synthesize the primers, although Operon had a
set of primers-only that was considerably cheaper than complete kits.
I also recieved some info on kits from Clontech, Genomyx, and
GenHunter--any comments on these?
The Clontech kit used one primer (poly-T) for Rev. Txn., which cut down
the number of reactions, while the other kits needed 4 separate Rev. txn
Again, any feedback on these or other kits would be appreciated.
More information about the Methods