How do you dissolve NBT and BCIP?

[Adrian Bracken]

Maybe it is because it WON'T EVER go into soln. Have you tried heating to
60 and vortexing well? Why DMF anyway? I know that I use the normal buffer
used to dissolve BCIP and NBT. Simply put in buffer3 (See Boeringer
mannheim DIG kit protocols. Buffer 3 is also called "detection buffer". It
is 100mM Tris-HCl, 100mM NaCl, pH 7.5). I hope this helps. Let me know if
that answers your question!, Adrian Bracken.

In article
<Pine.GSO.3.93.961216135323.18124A-100000 at lictor.acsu.buffalo.edu>,
xinguo at acsu.buffalo.edu wrote:

> Hi there,
> 
> I try to dissolve NBT and BCIP in  dimethylformamide to detect alkaline
> phosphatase activity. They just don't want to go into the solution. How do
> you do? Need quick responses. Thanks a lot.
> 
> Xin
> 
> 
> long axons and short dendrites      ~{VaM;3$!!JwM;6L~}
> http://www.acsu.buffalo.edu/~xinguo