suicide plasmid

Pascal Mertens Pascal.Mertens at
Tue Dec 17 07:01:47 EST 1996

In article (Dans l'article)
<s950142-1612962158040001 at>,
s950142 at wrote (écrivait) :

> Dear netters,
> I am going to construct a Salmonella mutant which may need to use  suicide
> plasmid, do you have any information about this matter? Thanks in advance
> for your help!

Suicide plasmid for a specific host is a plasmid that cannot replicate in
this host (that have no origin of replication  for this host). The only
way such a plasmid can maintain in the host is by specific or non-specific
recombination with the host's genome.

Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420

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