DNA software (Hybridization)

[WSchick at aol.com]

In a message dated 96-12-17 13:15:02 EST, you write:

<< 
 dlfeinstein wrote:
 
 >I am looking for a program (for mac or windows) that will allow me to
 determine annealing temps for simple DNA:DNA hybrids.However, what I
 need to do is to be able to detremine the effects of mutations on the
 Tm, e.g. single or double mismatches, different distances betwen two
 mistmatches, the effect of a single or double base insertion. Any good
 reference on basic annelaing temp theory would be extremely useful
  >>
I sell a program called the HybSimulator, which looks at all mismatches on
thermodynamic algorithms.  It is PC based, and will access Genbank or other
databases, or will run on subset databases that we can also provide.

Reference on the theory is M. Mitsuhashi, "Tech Report: Part 1. Basic
Requirements for Designing Optimal Oligo....", J Clin Lab Analysis 10:277-284
(1996).  There are more in the series on design philosophy for each
application, such as PCR QPCR, diagnostic probes, point-mutation dtetiion,
theraperutics, gene cloning, etc.

Please supply your mailing address and we will mail you information.

Walter Schick
ATR Biotech
Emeryville, CA