Concanavalin A immobilization

Marco Euler MEuler at
Wed Dec 18 19:57:05 EST 1996

I started my thesis work and I am trying to immobilize Con A via its
amine groups on glutaraldehyde modified Nylon matrix. The immobilization
procedure (reductive amination) requires a slightly basic pH (about 8).

No matter which buffer (with and without Ca2+ and Mn2+) I try, the conA
forms a turbid suspension.

As I am a lousy chemist, I am pretty stuck here. Has anybody had similar
experiences or any ideas?
Thanks a lot - and merry christmas,

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