RNAse showing up in gel after plasmid miniprep

[Paul N Hengen]

Marianne Leverone ", BIO (leverone at CHUMA.CAS.USF.EDU) wrote:

> Berrnard M:  We are using <1 ug/ml.  Only one person in our lab is having
> this trouble which is wierd.  We can't figure out why he is having such
> trouble!  He is now trying a phenol:chloroform then chloroform extraction
> to see if he can get rid of it.  this would be a pain because these are
> minipreps, and the templates (a lot of them) are being used for DNA
> sequencing (for mutation screening).  Marianne

Add MORE RNase. What you are seeing on the gel is the RNA and not RNase!

--
*******************************************************************************
* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
* Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
* Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
* Frederick, Maryland 21702-1201 USA              /--------------------------/*
* - - -  Methods FAQ list -> ftp://ftp.ncifcrf.gov/pub/methods/FAQlist - - -  *
* -  TIBS column archive -> http://www-lmmb.ncifcrf.gov/~pnh/readme.html - -  *
* - The BEST Molecular Biology HomePage -> http://www-lmmb.ncifcrf.gov/~pnh/  *
*******************************************************************************