RNAse showing up in gel after plasmid miniprep

Paul N Hengen pnh at ncifcrf.gov
Wed Dec 18 11:50:31 EST 1996


Marianne Leverone ", BIO (leverone at CHUMA.CAS.USF.EDU) wrote:

> Berrnard M:  We are using <1 ug/ml.  Only one person in our lab is having
> this trouble which is wierd.  We can't figure out why he is having such
> trouble!  He is now trying a phenol:chloroform then chloroform extraction
> to see if he can get rid of it.  this would be a pain because these are
> minipreps, and the templates (a lot of them) are being used for DNA
> sequencing (for mutation screening).  Marianne

Add MORE RNase. What you are seeing on the gel is the RNA and not RNase!

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