Ligation problems...

SARAH LOUISE RAWLINGS slr2 at coventry.ac.uk
Wed Dec 18 06:42:54 EST 1996


Could anyone please tell me why certain concentrations of ATP and the use 
of Hexammine coblalt (II) Chloride in blunt ended ligations are 
inhibitory to the ligation.
I'm having terrible problems ligating a blunted vector and DNA insert 
together!

I look forward to your replies..

SARAH 

Sarah Louise Rawlings       
Bsc (Hons) Biochemistry (Coventry University)
SLR2 at coventry.ac uk 


          
        
            




More information about the Methods mailing list