Removal of PCR DNA contamination

Andrew Atwell Andrew_Atwell at hp.com
Wed Dec 18 18:50:06 EST 1996


Brett Beitzel wrote:
> 
> In article <58rrg4$14e at mserv1.dl.ac.uk>, "John Morrow"
> <johnm at am.alba.umc.akzonobel.nl> wrote:
> 
> > Does anybody know of a good way to remove DNA contamination from surfaces by
> > chemical means, as opposed to UV.
> >
> > Cheers
> > John
> 
> I remember reading a Biotechniques article a while back that said that 10%
> bleach worked the best.  If you set up your PCRs in a hood, though, the
> bleach will corrode the metal (so I'm told.)
> 
> Cheers,
I've had luck with 1M HCL followed by 1M NaOH followed by 1X TBE
followed by H2O.  Good luck

-- 
Andrew Atwell			"Yes officer, I am aware that I was
Palo Alto, CA 			traveling at 120 m.p.h"  -Jetta VR6
Andrew_Atwell at hp.com



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