dna degradation

Karl Voss karl at hobbes.chem.ualberta.ca
Wed Dec 18 18:08:47 EST 1996

mobio at electriciti.com wrote:
> Does anyone know if there is a defined temperature which will break the 
> phosphor diester bond in the nucleic acid chain?

> Thanks in advance!!

Phosphodiester bonds are quite stable.  You can boil CCC dna solutions and
not create a nick in the backbone.  Even high pH will not destoy the backbone.
Now heating DNA with AP sites (apurinic/apyrimidinic) in a high pH solution
will break the phosphodiester backbone.  

Good luck.


If it ain't broke it doesn't have enough
features yet

Karl Voss
Department of Chemistry
University of Alberta
Edmonton, Alberta, Canada
T6G 2G2

karl.voss at ualberta.ca
phone 403-492-0222
fax   403-492-8231

More information about the Methods mailing list