dna degradation

Karl Voss karl at hobbes.chem.ualberta.ca
Wed Dec 18 18:08:47 EST 1996


mobio at electriciti.com wrote:
> Does anyone know if there is a defined temperature which will break the 
> phosphor diester bond in the nucleic acid chain?

> Thanks in advance!!

Phosphodiester bonds are quite stable.  You can boil CCC dna solutions and
not create a nick in the backbone.  Even high pH will not destoy the backbone.
Now heating DNA with AP sites (apurinic/apyrimidinic) in a high pH solution
will break the phosphodiester backbone.  

Good luck.

--
-------------------------------------------

If it ain't broke it doesn't have enough
features yet

-------------------------------------------
Karl Voss
Department of Chemistry
University of Alberta
Edmonton, Alberta, Canada
T6G 2G2

karl.voss at ualberta.ca
http://hobbes.chem.ualberta.ca/~karl
phone 403-492-0222
fax   403-492-8231



More information about the Methods mailing list