Concanavilin A immobilization

Kurt L Brillhart klbrillhart at ccgate.dp.beckman.com
Tue Dec 17 09:50:32 EST 1996


Unlike most coupling chemistries, the aldehyde you are using 
works well under a broad range of pH conditions.  I would suggest coupling at 
neutral or even slightly slightly acid pH.  Varying the salt concentration may 
help also.  BTW- you might want to consider coupling in the presence of ligand 
in order to protect the binding site.

Kurt Brillhart



More information about the Methods mailing list