salmon sperm DNA

Alexander N. Kukushkin kan at mmcd.cyt.ras.spb.ru
Thu Dec 19 12:00:53 EST 1996


>   Does anyone know how to properly prepare salmon sperm DNA?
>
>   Manniatus suggests to dissolve it to 10mg/ml, shear it, then extract
>   it with phenol/chloroform.  Shearing it is no problem but i tried to
>   extract it and it just looked like glue. After spinning it to seperate
>   the phases, the aqueous layer looked terrribleand very cloudy.  I'm
>   sure it is way too viscous and too conc'd to extract.  Therefore can
>   you get by without extracting? or what do I need to do.
>
>   Ps we will be using this for cell culture transfections and as a
>   blocking agent for blots.
>
>   darren
>
Hi, Darren
You can try the following way. Sonicate the DNA solution and then extract it.
Also it is easy to dilute it before extraction.
Good luck
Alex





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