salmon sperm DNA
Alexander N. Kukushkin
kan at mmcd.cyt.ras.spb.ru
Thu Dec 19 12:00:53 EST 1996
> Does anyone know how to properly prepare salmon sperm DNA?
> Manniatus suggests to dissolve it to 10mg/ml, shear it, then extract
> it with phenol/chloroform. Shearing it is no problem but i tried to
> extract it and it just looked like glue. After spinning it to seperate
> the phases, the aqueous layer looked terrribleand very cloudy. I'm
> sure it is way too viscous and too conc'd to extract. Therefore can
> you get by without extracting? or what do I need to do.
> Ps we will be using this for cell culture transfections and as a
> blocking agent for blots.
You can try the following way. Sonicate the DNA solution and then extract it.
Also it is easy to dilute it before extraction.
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