Cocktail sticks and toothpicks supplier

Dr. Duncan Clark duncan at
Wed Dec 18 09:26:09 EST 1996

In article <596faf$8ps6 at>, Paul N Hengen
<pnh at> writes
>So, nobody can really confirm that wooden toothpicks are a problem for
>screening colonies by PCR????

We've used the Kaybee ones for a lot of colony PCR's (must be in excess
of a thousand) and have never had problems. We autoclave them in 30ml
glass universals then use them. 

One minor problem. I did some colony PCRs in Hong Kong a few months ago.
The lab had dried their toothpicks at high temperature overnight after
autoclaving. I picked up a new batch, now cool, and gridded a plate. I
left the toothpicks sitting in 20ul water, which I normally don't do,
prior to a normal quick mix and heating at 100C for 5mins. They acted
like blotting paper and soaked up the 20ul in a couple of minutes flat.
So don't dry them too much then leave them sitting in water! All was not
lost as addition of a further 20ul, a quick mix etc and the PCR's worked


The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Dr. Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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