aluI digestion problem

medbh at biovax.leeds.ac.uk medbh at biovax.leeds.ac.uk
Thu Dec 19 08:49:51 EST 1996


Has anyone had any problems digesting with aluI? I have had problems with partial digestions of PCR products with this enzyme. I have tried various concentrations of the enzyme, and purifiying my samples through spin columns, but have not got it to properly work. Has anyone any suggestions?



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