Protein sticking to glass..

Dennis Summerbell d-summer at nimr.mrc.ac.uk
Thu Dec 19 06:48:21 EST 1996


In article <59b19r$ist at mserv1.dl.ac.uk>, Wolfgang Schechinger
<wgschech at med.uni-tuebingen.de> wrote:

> >(a) How serious is this problem? i.e. Is the percentage of protein
> >sticking to glass quite high?
> That depends on the protein
> 
> 
> >(b) How can one avoid this problem?

If your unconcerned about a little extraneous protein contamination,  the
simplest method is to rinse the glassware in a 1% solution of BSA in the
buffer that you are using, then rinse a couple of times in buffer alone.
This is also useful to avoid sticking cells or tissues to glass.

Dennis

Dr D. Summerbell, NIMR, The Ridgeway, Mill Hill, London NW7 1AA.

email: d-summer at nimr.mrc.ac.uk
Tel: +181 959 3666 X2366
Fax: +181 913 8527



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