Protein sticking to glass..
d-summer at nimr.mrc.ac.uk
Thu Dec 19 06:48:21 EST 1996
In article <59b19r$ist at mserv1.dl.ac.uk>, Wolfgang Schechinger
<wgschech at med.uni-tuebingen.de> wrote:
> >(a) How serious is this problem? i.e. Is the percentage of protein
> >sticking to glass quite high?
> That depends on the protein
> >(b) How can one avoid this problem?
If your unconcerned about a little extraneous protein contamination, the
simplest method is to rinse the glassware in a 1% solution of BSA in the
buffer that you are using, then rinse a couple of times in buffer alone.
This is also useful to avoid sticking cells or tissues to glass.
Dr D. Summerbell, NIMR, The Ridgeway, Mill Hill, London NW7 1AA.
email: d-summer at nimr.mrc.ac.uk
Tel: +181 959 3666 X2366
Fax: +181 913 8527
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