Suggestions for vacuum pump
jkropp at itsa.ucsf.edu
Thu Dec 19 19:59:07 EST 1996
In article <597dl2$6vu at sjx-ixn5.ix.netcom.com>, sendero at ix.netcom.com(Dr.
Michael T. MacDonell) wrote:
> In <597cdt$9qi$1 at news-s01.ca.us.ibm.net> arkcon at ibm.net writes:
> >HI all. I want to buy a vacuum pump for a small private research lab.
> >to use it to degass acrylamide and other solutions,
> You know, you could approach the problem the other way round,
> and buy a small tank of nitrogen or argon and bubble it through
> the mixture.
There is no need to degas acrylamide if you use an excess of APS (this
should be in a FAQ. "It is? Damn!")
I use 90ul 25% APS for 60 ml sequencing gel, and never degas.
And, no, I haven't titrated the stuff, smartypants.
"no brains...no headache!"
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