Suggestions for vacuum pump

Jerry Kropp jkropp at itsa.ucsf.edu
Thu Dec 19 19:59:07 EST 1996


In article <597dl2$6vu at sjx-ixn5.ix.netcom.com>, sendero at ix.netcom.com(Dr.
Michael T. MacDonell) wrote:

> In <597cdt$9qi$1 at news-s01.ca.us.ibm.net> arkcon at ibm.net writes: 
> >
> >HI all.  I want to buy a vacuum pump for a small private research lab. 
I want
> >to use it to degass acrylamide and other solutions,
> 
> You know, you could approach the problem the other way round, 
> and buy a small tank of nitrogen or argon and bubble it through 
> the mixture. 
> 
> -Mike
> 
Hi Guys,
There is no need to degas acrylamide if you use an excess of APS (this
should be in a FAQ. "It is? Damn!")
I use 90ul 25% APS for 60 ml sequencing gel, and never degas. 
And, no, I haven't titrated the stuff, smartypants.
Good luck, 
Jerry

-- 
"no brains...no headache!"



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