Blood Storage for DNA Extraction

Jerry Kropp jkropp at itsa.ucsf.edu
Thu Dec 19 19:50:34 EST 1996


In article <32B72C36.5008 at mnw.net>, Rick Bright <rbright at mnw.net> wrote:

> I have been successfully extractin DNA from frozen blood (-70C) but
> today, for some reason, nothing worked out.  The blood was all collected
> in the second week of November.  While it was fresher, it had a great,
> pure yield.  Now, just garbage, after about 5-6 weeks of storage.
> 
> Does anyone know the length of stability for frozen blood for DNA
> extraction.  I have obviously crossed the line, but just how far?  Guess
> I will have to roll up my sleeves again tomorrow.
> 
> Rick
> rbright at mnw.net

Hi Rick,
I've extracted restriction and PCR-competent DNA from whole blood that has
been stored at -20 for more than a year. In fact, I've never used -70.

Methinks something else went wrong.

Good Luck,
Jerry

-- 
"no brains...no headache!"



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