aluI digestion of PCR products

Alex Dobrovic adobrovic at medicine.adelaide.edu.au
Thu Dec 19 19:42:40 EST 1996


>Date: Fri, 20 Dec 1996 10:15:32 +0930
>To:medbh at biovax.leeds.ac.uk
>From:adobrovic at medicine.adelaide.edu.au (Alex Dobrovic)
>Subject:Re: aluI digestion problem
>
>>Has anyone had any problems digesting with aluI? I have had problems with
>>partial digestions of PCR products with this enzyme. I have tried various
>>concentrations of the enzyme, and purifiying my samples through spin
>>columns, but have not got it to properly work. Has anyone any
>>suggestions?
>
>It may be that you have a heteroduplex problem? If you are digesting a
>sample with an Alu1 polymorphism, the undigested band will always be
>stronger. Full digestion will be seen when you digest a homozygote that
>has an Alu1 site on both alleles.
>

Alexander Dobrovic, Ph. D.
Chief Scientist
Department of Haematology-Oncology
The Queen Elizabeth Hospital
Woodville, SA 5011, Australia

Affiliate Senior Lecturer
Department of Medicine
University of Adelaide

Tel 61-8-8222 6884
Fax 61-8-8222 6046 (note new numbers as of August 1996)





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