Cloning Double-Standed Oligos

Lourdes Rodgers lrsdr at
Thu Dec 19 15:04:58 EST 1996

If anyone has experience with cloning double stranded oligos please send 
me useful tips such as: should the primers be phosphorylated?, tips on 
annealing primers, ligation reaction conditions, etc. 

Lourdes Rodgers
Graduate student
The University of Michigan
Ann Arbor MI

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