Anomalous PCR product

[Nico van Belzen]

Looks as if the 600 bp product has the first oligo on both ends. The 
amplification in the absence of target DNA suggests contamination of pre-PCR 
reagents with the 600 bp product.

Best regards, Nico


>>        I have used an oligo for PCR which resulted in a major,
>unexpected
>>product of 600 bp.  The same  600 bp product was generated when no
>second primer >and no template DNA was put through the "PCR".  Do you
>have any experience to >suggest what might be causing this?