Anomalous PCR product
Nico van Belzen
belzen at hema.fgg.eur.nl
Fri Dec 20 09:30:46 EST 1996
Looks as if the 600 bp product has the first oligo on both ends. The
amplification in the absence of target DNA suggests contamination of pre-PCR
reagents with the 600 bp product.
Best regards, Nico
>> I have used an oligo for PCR which resulted in a major,
>>product of 600 bp. The same 600 bp product was generated when no
>second primer >and no template DNA was put through the "PCR". Do you
>have any experience to >suggest what might be causing this?
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