Neomycin resistance

LABO DE BIOCHIMIE lbmc at sciences.univ-metz.fr
Fri Dec 20 14:03:26 EST 1996


Hi all.
I am planing to use E.coli strain NM522 transformed with the pCI-neo
plasmid (Promega). Could anybody help me in giving the procedure
(neomycin concentration, transformation...) for selecting transformants
on neomycin-containing medium plates? Does this strategy require special
care to any step?
Thanks a lot.
Mark.



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