quick hybidizations

Michael W. Thompson mthom0 at pop.uky.edu
Fri Dec 20 11:53:37 EST 1996


anat-hd wrote:
> 
> Sorry to correct you, but colonies contain an afwul lot of target
> DNA, and the southerns that you mention are presumably NOT
> genomic southerns, but PCR or restriction frags ?
> 
> Depending on WHAT you want to detect, such short hyb times are
> not extraordinary.... However, in the buffer that you describe
> a genomic southern would still take at least 16 h....
> 
> BTW, leave out the Denhardt's and use 7% SDS: easier
> and less background.
> 
> So anybody know the recipe of a true quick buffer ?
> 
> clemens

	Likewise, does anyone know what the "express-hyb" Northern blot buffer
is, offhand?

Michael Thompson
Dept. of Biochemistry
University of Kentucky
Lexington, KY 40536-0084



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