blunt end !!!

[George Rutherford]

In article <59ddcr$bnk at mserv1.dl.ac.uk>, Wolfgang Schechinger
<wgschech at med.uni-tuebingen.de> wrote:

> isabella, did you phosphorylate your insert or the cut up vector?
> 
> merry x-mess
> Wolfgang
> 
> At 02:53 20.12.1996 GMT, you wrote:
> >Help!Help!Help!
> >I have tried blunt end ligation for two mouths.
> >But the cloning was still not done.
> >Does anybody have good method?
> >Please help me!
> >Thanks.
> >                        isabella Chen
> >
> >
> >
Why would anyone phosphorylate either component? One might consider CIPing
the vector, and maybe polishing the ends.

Geo