blunt end !!!
gruther at bilbo.bio.purdue.edu
Fri Dec 20 11:31:54 EST 1996
In article <59ddcr$bnk at mserv1.dl.ac.uk>, Wolfgang Schechinger
<wgschech at med.uni-tuebingen.de> wrote:
> isabella, did you phosphorylate your insert or the cut up vector?
> merry x-mess
> At 02:53 20.12.1996 GMT, you wrote:
> >I have tried blunt end ligation for two mouths.
> >But the cloning was still not done.
> >Does anybody have good method?
> >Please help me!
> > isabella Chen
Why would anyone phosphorylate either component? One might consider CIPing
the vector, and maybe polishing the ends.
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