Plasmid contamination from pipettors

Dr. Duncan Clark duncan at
Sat Dec 21 07:51:49 EST 1996

In article <32BAC571.D7B at>, "Michael W. Thompson"
<mthom0 at> writes
>Does anyone out there know of a good procedure to decontaminate
>pipettor barrels for use in RT-PCR experiments and likewise?  A big
>problem in our lab is that since we have been working so extensively
>with specific cDNAs, when we try to run an experiment such as RT-PCR
>which is sensitive to contamination, there are multiple bands in all
>       Anyone?
>Michael Thompson
>Dept. of Biochemistry
>University of Kentucky
>Lexington, KY 40536-0084

Some pipette barrels are autoclavable. Mild bleach - 10%? Use filter
pipette tips. Are you sure your problem is the barrels and not just
general background lab contamination. If you make up your PCR's in a
non-DNA lab with your pipettes do you still get contamination?  


The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Dr. Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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