RNase H

Lourdes Rodgers lrsdr at umich.edu
Mon Dec 23 10:54:41 EST 1996

Can anyone tell me what is the advantage of using RNase H in an RNase 
Protection Assay?  I have been using RNase A and T1 but I still have 
background bands that are not being digested away.  The are also present 
in the yeast RNA control lane.  Can someone offer some help?  Thanks, 
Lourdes Rodgers
The University of MIchigan
Ann Arbor, MI

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