salmon sperm DNA
Marieke R. Koedood Zhao
rkoedood at bio.bu.edu
Mon Dec 23 14:17:55 EST 1996
We don't extract either - we dissolve the DNA, shear it by pulling through a
syringe needle multiple times and then boil it for 10 min. That's it.
Works fine for hybridizations as well as yeast transfections.
darren cikaluk (dcikaluk at anatomy.anat.med.ualberta.ca) wrote:
: Does anyone know how to properly prepare salmon sperm DNA?
: Manniatus suggests to dissolve it to 10mg/ml, shear it, then extract
: it with phenol/chloroform. Shearing it is no problem but i tried to
: extract it and it just looked like glue. After spinning it to seperate
: the phases, the aqueous layer looked terrribleand very cloudy. I'm
: sure it is way too viscous and too conc'd to extract. Therefore can
: you get by without extracting? or what do I need to do.
: Ps we will be using this for cell culture transfections and as a
: blocking agent for blots.
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