PCR with Pwo DNA polymerase from Boehringer

Paul N Hengen pnh at ncifcrf.gov
Tue Dec 17 11:53:32 EST 1996


vilimf01 at mcrcr6.med.nyu.edu wrote:

: If the Barnes paper isn't part of the FAQ list, it ought to be.
: (WM Barnes. PCR amplification of up to 35-kb DNA with high fidelity and
: high yield from lambda bacteriophage templates. PNAS 91: 2216-2220
: (1994).)  The basic idea is that....<snip>

The question was about the use of Pwo polymerase from Boehringer not
the Taq-Pfu mixtures described by Wayne Barnes. Although the FAQ list
does not have this in it, an entire column in TIBS was dedicated to
the subject and even has a reply from Wayne Barnes about some common
problems encountered with the technique. The article is available from
my Homepage.

@article{Hengen1994Augtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Long and accurate {PCR}",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "8",
pages = "341-342",
month = "aug",
year = "1994"}

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