Dr E. Buxbaum
EB15 at le.ac.uk
Tue Dec 17 11:38:42 EST 1996
brackena at tcd.ie (Adrian Bracken) wrote:
>Hi, I hope to add an enzyme which transiently interacts with its target
>protein(s). I have antibodys for the enzyme and I have prepared a column
>with these antibodies. I plan to run extracts through it. If I can somehow
>link the enzyme to its target(s), prior to running on column, I should be
>able to purify the enzymes targets??!!
You need a bifunctional crosslinker for this. Check the Pierce catalog
for a good selection of such substances.
One other thing though: Do not expect miracles. Crosslinking is a
notoriously "dirty" method.
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