salmon sperm DNA
J. Pat Martinez
martinez at puccini.CRL.umn.edu
Sat Dec 21 06:08:10 EST 1996
We dissolve it (from Sigma) in water then autoclave the solution for 15 -
20 minutes to shear it (much easier than using a sonicator). We don't
bother to extract it with organics. Works fine in our prehyb. soln.
In article <32B7F7AD.43BC at anatomy.anat.med.ualberta.ca>, darren cikaluk
<dcikaluk at anatomy.anat.med.ualberta.ca> wrote:
> Does anyone know how to properly prepare salmon sperm DNA?
> Manniatus suggests to dissolve it to 10mg/ml, shear it, then extract
> it with phenol/chloroform. Shearing it is no problem but i tried to
> extract it and it just looked like glue. After spinning it to seperate
> the phases, the aqueous layer looked terrribleand very cloudy. I'm
> sure it is way too viscous and too conc'd to extract. Therefore can
> you get by without extracting? or what do I need to do.
> Ps we will be using this for cell culture transfections and as a
> blocking agent for blots.
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