Ligation problems...

Chen Ho An chen at bsm.bioc.ucl.ac.uk
Mon Dec 23 07:22:08 EST 1996


George Rutherford (gruther at bilbo.bio.purdue.edu) wrote:
: In article <Pine.ULT.3.91.961218114009.21727A-100000 at rowan>, SARAH LOUISE
: RAWLINGS <slr2 at coventry.ac.uk> wrote:

: > Could anyone please tell me why certain concentrations of ATP and the use 
: > of Hexammine coblalt (II) Chloride in blunt ended ligations are 
: > inhibitory to the ligation.
: > I'm having terrible problems ligating a blunted vector and DNA insert 
: > together!
: > 

: Sarah



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