Ligation problems...

[Chen Ho An]

George Rutherford (gruther at bilbo.bio.purdue.edu) wrote:
: In article <Pine.ULT.3.91.961218114009.21727A-100000 at rowan>, SARAH LOUISE
: RAWLINGS <slr2 at coventry.ac.uk> wrote:

: > Could anyone please tell me why certain concentrations of ATP and the use 
: > of Hexammine coblalt (II) Chloride in blunt ended ligations are 
: > inhibitory to the ligation.
: > I'm having terrible problems ligating a blunted vector and DNA insert 
: > together!
: > 

: Sarah