Protease Inhibitor Cocktails

Richard C. Padgett richard-padgett at
Tue Dec 17 11:33:26 EST 1996

I would be interested in how people are storing their protease inhibitors
for use in Western and IP blots.  In particular, do people mix up and
freeze aliquots of a cocktail or do you make it up fresh each time.
The back of the Pharmingen 96/97 give the following cocktail recipe.

100X Protease Inhibitor Cocktail

Benzamidine HCL	1.6 mg/ml
Phenanthroline		1.0 mg/ml
Aprotinin		1.0 mg/ml
Leupeptin		1.0 mg/ml
Pepstatin A		1.0 mg/ml
PMSF			100 mM
Dissolve in 100% Ethanol

It would seem to me that this would be perfect to aliquot and freeze for
future use.

Richard Padgett
Division of Cardiovascular Disease
Univ. of Iowa School of Medicine

More information about the Methods mailing list