RNase H

[Jillian Johnston]

Doesn't RNaseH digest RNA-DNA hybrids?  If so, this would cause
your protected RNA fragmant to disappear and so much for your
RPA! 
Lourdes Rodgers (lrsdr at umich.edu) wrote:
: Can anyone tell me what is the advantage of using RNase H in an RNase 
: Protection Assay?  I have been using RNase A and T1 but I still have 
: background bands that are not being digested away.  The are also present 
: in the yeast RNA control lane.  Can someone offer some help?  Thanks, 
: Lourdes Rodgers
: The University of MIchigan
: Ann Arbor, MI

--
Jillian Johnston
Dept. of Biological Sciences       Guider
Univ. of Calgary         	   30 Pathfinders, Tanisi District