Q: Flow cytometry with anti-flag?

Reuven Stein reuvens at ccsg.tau.ac.il
Wed Dec 25 16:11:56 EST 1996


Hi!
Does anyone have any experience with flow cytometry analysis (FACS)
using an anti-FLAG antibody? I intend to add this flag (8 aa) to a 7
transmmembrane protein which is localized to inner membranes (not plasma
membrane), and then use this flag to select cells (with a FACS).
All sugestions will be grately appreciated.
Thanks,
Amir Gamliel 
e-mail: a657109 at post.tau.ac.il
Dept. of Neurobiochemstry
Tel-Aviv University
ISRAEL



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