Heat inactivation of the phosphor diester bond in DNA

Anton Tutter atutter at jeeves.ucsd.edu
Thu Dec 26 18:07:09 EST 1996

mobio at electriciti.com wrote:
> Does anyone know of a specified temperature which will permanently break the
> phosphor diester bond in the backbone of nucleic acid??????????????

As far as I know, boiling temp. will not cleave the phosphodiester bond in 
DNA.  Why don't you try low pH (< 3).  This bond is unstable at low pH.

Anton Tutter
Salk Institute
La Jolla, CA

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