Heat inactivation of the phosphor diester bond in DNA

[Anton Tutter]

mobio at electriciti.com wrote:
> 
> Does anyone know of a specified temperature which will permanently break the
> phosphor diester bond in the backbone of nucleic acid??????????????
> 


As far as I know, boiling temp. will not cleave the phosphodiester bond in 
DNA.  Why don't you try low pH (< 3).  This bond is unstable at low pH.


Anton Tutter
Salk Institute
La Jolla, CA