Heat inactivation of the phosphor diester bond in DNA
atutter at jeeves.ucsd.edu
Thu Dec 26 18:07:09 EST 1996
mobio at electriciti.com wrote:
> Does anyone know of a specified temperature which will permanently break the
> phosphor diester bond in the backbone of nucleic acid??????????????
As far as I know, boiling temp. will not cleave the phosphodiester bond in
DNA. Why don't you try low pH (< 3). This bond is unstable at low pH.
La Jolla, CA
More information about the Methods