Help on Agarose!
Eike Christian Buss
E.Buss at DKFZ-Heidelberg.de
Fri Dec 27 17:41:36 EST 1996
> Hi, I was wondering if anyone has the protocol for making agarose? I have
> search endlessly over the net with little luck.
> Thanks in advance
> Cindy Lin
I wonder what exactly your problem is,
Do you want to prepare Agarose right from the source, from some alga from
the southsea? In this case I cannot help you.
If you just want to prepare it: 1. buy Agarose from almost any supplier
2. mix it in 1-2% solution ( depending on what you want to do with it)
with your buffer solution (TBE e.g.)
3. Heat it up in a waterbath or faster in a microwave oven until it boils
4. Let it cool down until you can pour it into your gel tray.
Tell me if you need more hints on this
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