degenerate oligos: I or everything?

jstrassw at OPAL.TUFTS.EDU jstrassw at OPAL.TUFTS.EDU
Sat Dec 28 16:16:54 EST 1996

I would like to know what the preferrred oligo strategy is for reverse
genetics.  I have a peptide sequence and would like to PCR out the
corresponding cDNA.  So, I have two choices: either put inosine in the 3rd
position or include all 4 bases at the redundant position.  Any thoughts?

John Strasswimmer, MD,PhD Candidate    | Phone (617) 636 8396
Tufts - New England Medical Center     | Fax   (617) 636 6190
Box 166                                | Email:  jstrassw at
Boston, MA 02111 USA                   |                              
     *** Co-Author of "HyperBug" Microbiology Teaching Software ***
Remember, Your employer is eavesdropping...  

More information about the Methods mailing list