degenerate oligos: I or everything?

jstrassw at OPAL.TUFTS.EDU jstrassw at OPAL.TUFTS.EDU
Sat Dec 28 16:16:54 EST 1996


Hello!
I would like to know what the preferrred oligo strategy is for reverse
genetics.  I have a peptide sequence and would like to PCR out the
corresponding cDNA.  So, I have two choices: either put inosine in the 3rd
position or include all 4 bases at the redundant position.  Any thoughts?
Thanks,
John


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John Strasswimmer, MD,PhD Candidate    | Phone (617) 636 8396
Tufts - New England Medical Center     | Fax   (617) 636 6190
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