Removal of E.coli non-specific binding

DAVID PULFORD david.pulford at ukonline.co.uk
Mon Dec 30 14:59:08 EST 1996


I'm looking for a quick and easy way of removing E.coli antibodies from a
rabbit serum. I've found pseudoscreening an incomplete, time consuming and
expensive way of removing this background activity for screening an E.coli
expression library. I chanced upon a method a while back that used
formalised cells but have no reference for this approach. Can anyone help?

Thanks DJP



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