how to remove SDS from RNA sample?

brett brett at BORCIM.WUSTL.EDU
Mon Dec 30 12:10:41 EST 1996


>Seng-Lai Tan <sltan at u.washington.edu> wrote:
>
>>Dear fellow researchers,
>
>>i'm trying to reverse transcribe my RNA but it's resuspended in water that
>>contains some SDS, which is inhibiting the RT reaction.  Does anyone know
>>of a easy method (e.g. precipitation) that I can use to remove the SDS in
>>my sample?  many thanks in advance.
>
>>seng-lai
>
>
>I try not to intrude on scientific discussions in methds-reagnts with
>commercial interests.  However, there are times when it is appropriate
>to let the scientific community know that there are products which may
>not be commonly known that can solve their problems.  This is such a
>case.  We have a  product available which is able to remove SDS from
>alkaline lysis samples as well as other more purified samples.  Let me
>know if you desire more information.
>
>Regards,
>
>John

Thanks John, for sparing us. I have another idea using commercially available
products --- organic extraction, such as phenol.


Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             




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