how to remove SDS from RNA sample?
brett at BORCIM.WUSTL.EDU
Mon Dec 30 12:10:41 EST 1996
>Seng-Lai Tan <sltan at u.washington.edu> wrote:
>>Dear fellow researchers,
>>i'm trying to reverse transcribe my RNA but it's resuspended in water that
>>contains some SDS, which is inhibiting the RT reaction. Does anyone know
>>of a easy method (e.g. precipitation) that I can use to remove the SDS in
>>my sample? many thanks in advance.
>I try not to intrude on scientific discussions in methds-reagnts with
>commercial interests. However, there are times when it is appropriate
>to let the scientific community know that there are products which may
>not be commonly known that can solve their problems. This is such a
>case. We have a product available which is able to remove SDS from
>alkaline lysis samples as well as other more purified samples. Let me
>know if you desire more information.
Thanks John, for sparing us. I have another idea using commercially available
products --- organic extraction, such as phenol.
Program in Immunology
Washington University - St Louis
brett at borcim.wustl.edu
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