removing e.coli non-specific activity from antisera

[sharmaa at acsu.buffalo.edu]

DAVID PULFORD wrote:
> 
> Hi,  I'm looking for a rapid technique I might employ for removing
> background non-specific activity from rabbit sera to E.coli proteins to
> screen a fusion protein library. I've tried psuedoscreening with wildtype
> bacteria on filters but have found it laborious and expensive in time and
> materials.  I read a method recently that employed formalised bacteria that
> was robust and quick but cannot find the reference again. Can anybody help
> with ideas or a citation of this method.  Thanks.
> 
> dave p

Dave, I don't know which article you are looking for but a protocol 
that utilizes a similar approach to adsorb out non-specific antibodies 
to E.coli proteins appeared in Biotechniques vol 19 No.1 (1995).  I 
used this procedure quite successfully to screen expresion libbaries.
Good Luck and Happy New Year.  
-- 
Name: Ashu Sharma
Tel: (716) 829-3518
Fax: (716) 829-3942                       
E-mail: sharmaa at acsu.buffalo.edu