Methods December 1996 Archives by author

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Starting: Sun Dec 1 06:48:46 EST 1996
Ending: Tue Dec 31 19:39:21 EST 1996
Messages: 810

RNAse showing up in gel after plasmid miniprep "Marianne Leverone ", BIO
RNAse showing up in gel after plasmid miniprep "Marianne Leverone ", BIO
"reverse northern" problems Gregory S. Buzard 'Buz'
Req: Best transformation protocol for LCL's *
Inserting 30 nt - which method? - help
degenerate oligos: I or everything?
Plasmid wanted: PiVX A.Habel
testing ADokun at gnn.com
Bailey's Irish Cream Achim Recktenwald, PhD
WANTED: PEPTIDE SYNTHETASE DOMAINS David Ackerley
DNA yield from plant tissue. Michael Adamson
NUCLEAR RUN-ON ASSAY Dr Alok Adholaya
Silver Staining of DNA (fwd) Dr Alok Adholaya
NUCLEAR RUN-ON ASSAY Dr Alok Adholaya
REP PRIMER Dr Alok Adholaya
DDRT-PCR Dr Alok Adholaya
CuraGen Corp Information Dr Alok Adholaya
DDRT-PCR Dr Alok Adholaya
SILVER STAINING Dr Alok Adholaya
RNA STAINING Dr Alok Adholaya
DYNAL BEADS Dr Alok Adholaya
RNA ligase Dr Alok Adholaya
BIOSCI/bionet miniFAQ & Fundraiser BIOSCI Administrator
cDNA library amplification M. Aitchison
Plasmid wanted: PiVX Ashok Aiyar
blunt ligation Ashok Aiyar
Neomycin resistance Ashok Aiyar
Two hybrid library from Jurkat or HEK293 cells? Ashok Aiyar
VIRUS WARNING Ashok Aiyar
What makes gels smile? Jens Alfken
Storing RNA Ghis Allard
tet- free serum Danny Altschuler
tet- free serum Danny Altschuler
Ligation problems... Chen Ho An
Protease Inhibitors Christophe Andreoli
What makes gels smile? Curt Ashendel
On again off again PCR Andrew Atwell
Removal of PCR DNA contamination Andrew Atwell
Primer design software Andrew Atwell
CSPD and blocking your blots Dave B
Neomycin resistance LABO DE BIOCHIMIE
How to measure total protein conc. of cultured-cell lysate? Mohamed BaSalamah
How to measure total protein conc. of cultured-cell lysate? Mohamed BaSalamah
sensitive, stable transcriptional markers? L. Bastian
How to discriminate male and female? Cousin Beatrice
Lambda DNA extraction protocol Robin Beech
His Tag Expression Vector Eric Beitz
Removal of PCR DNA contamination Brett Beitzel
SDS-PAGE SAMPLES Tom Belbin
?? Kinetics Software - Post Brad Bell
Anomalous PCR product Nico van Belzen
bacteriophage f1 Michael Benedik
pTZ18R Deborah Bernsen
(none) Laboratorio Bioclon
Differential Display: best kit/primers? Display Systems Biotech
Phage display vector request. Display Systems Biotech
purification of His-tagged proteins Volker Blaschke
T/A PCR cloning Holger Bohnemeier
(no subject) Yves Boisclair
bovine and liver cDNA library Yves Boisclair
site spesific integration Alex Borovkov
thrombin Joan Boyce
Cocktail sticks and toothpicks supplier Chris Boyd
Protein sequencing ??!! Adrian Bracken
Protein sequencing ??!! Adrian Bracken
x-linking protein interactions? Adrian Bracken
How do you dissolve NBT and BCIP? Adrian Bracken
X-linking proteins? Adrian Bracken
Nde I restriction enzyme John Brennand
Lambda DNA extraction protocol John Brennand
Storing RNA John Brennand
Microwave sterilization John Brennand
denaturing DNA in 0.12Mpb John Brennand
RT PCR woes... John Brennand
Method to extract DNA from yeasts Kenneth Briffa
Plant DNA Extraction Rick Bright
Plant Prep for DNA Extraction Rick Bright
Blood Storage for DNA Extraction Rick Bright
Help Rick Bright
Help Rick Bright
Help Rick Bright
DNA purity levels Rick Bright
Protein Purification Help Kurt L Brillhart
Concanavilin A immobilization Kurt L Brillhart
Scientists: tell me about your web site Megan Brown
Differential Display: best kit/primers? Paul A Bucciaglia
Microwave sterilization Sebastian Bunka
Phenol extraction problem/phases not separating Bill Burnett
Cocktail sticks and toothpicks supplier Bill Burnett
How long will labelled oligos last? Bill Burnett
Help on Agarose! Eike Christian Buss
Bailey's Irish Cream Dr E. Buxbaum
What makes gels smile? Dr E. Buxbaum
Bailey's Irish Cream Dr E. Buxbaum
composition of the ER lumen Dr E. Buxbaum
Removing the lipid from the protein sample Dr E. Buxbaum
Protein Purification Help Dr E. Buxbaum
X-linking proteins? Dr E. Buxbaum
RTPCR to PCR C.R.Baker
Lysis of bacteria in maxi prep. Florence CABON
nt2 / hnt2 cell question Lyngine Calizo
Protein Purification Help Jim Campanella
proteins methylation POSTEL Caroline
Source for 18S and/or 28S rat/mouse ribosomal seq Edmundo Castro
RNase removal by phenol-chloroform ? Edmundo Castro
Micronuclei Staining Cell.Morphology at rz.ruhr-uni-bochum.de
NB4 expression library Nicola Celli
Protease Cleavage George W. Chacko
Blund ends cloning Alex Chang
blunt ligation Chang,jihun
I'm sorry Chang,jihun
pBluescript v. pBluescriptII ?? Mic Chaudoir
pBluescript v. pBluescriptII ?? Joe Chou
Mutagenesis Kits Joe Chou
PCR of a 4.9 kb fragment Christophe
How to wash glassware for using with mRNA experiment Shi-Jye Chu
gel smear without stop Shi-Jye Chu
Quality Sticks Dr. Duncan Clark
Automatic sequence analyzer??? Dr. Duncan Clark
Cocktail sticks and toothpicks supplier Dr. Duncan Clark
Bp-exchange by PCR Dr. Duncan Clark
What makes gels smile? Dr. Duncan Clark
How long will labelled oligos last? Dr. Duncan Clark
Anomalous PCR product Dr. Duncan Clark
Cocktail sticks and toothpicks supplier Dr. Duncan Clark
Need sequence reformatting program for DNASIS Dr. Duncan Clark
Help Dr. Duncan Clark
Plasmid contamination from pipettors Dr. Duncan Clark
NspB II source? Dr. Duncan Clark
0.75 mm spacers and comb Robert A. Clark
Baileys Irish Cream David Clarke
MitoProt, a software for predictiong mitochondrial transit peptides Manuel G. Claros
How to dissolve NBT and BCIP ---> not in DMFA ! Dr. Clemens
DNA software Dr. J.P. Clewley
RNase removal by phenol-chloroform ? Steven Cohen
Glutathione Magnetic Beads Steven Cohen
Help: Transcription & Translation Errol Colak
native vs denaturing gel for RPA Shelley Cole
Bailey's Irish Cream Robin Colgrove
Request: Human B or T cell cDNA bank Dr. V Collins
Haplotype analysis Martin Collinson
Why does in situ work with sense probes, too? Martin Collinson
Plasmid contamination from pipettors Martin Collinson
Anomalous PCR product The Great American Gene Company
Weak Genebank Searches? Peter S. Cooper
Questions on the RNA extraction Peter S. Cooper
hybriZAP Seth Corey
gcg Joao Vasconcelos Costa
gcg Joao Vasconcelos Costa
nih image-help- Elias Coutavas
tet- free serum Robert Cowherd
Nde I restriction enzyme Robert Cowherd
how to remove SDS from RNA sample? Robert Cowherd
denaturing DNA in 0.12Mpb Andrea Crampton
DNA Fragments from Eukaryotic Cells Neil Crickmore
electroporation and ion-channel currents Daniel Cross
E. coli mutant wanted Richard Daines
gcg Daniel.Marc
Lamda Sequencing... David L. Haviland, Ph.D.
RNAse showing up in gel after plasmid miniprep David L. Haviland, Ph.D.
NspB II source? Jenny Davila-Aponte
Human IL-3 Assay Greg Davis
phosphotyrosine peptides Mike Daws
Liposomes from cells? Mike Daws
HELP! TBS for GST? Michael DiDonato
GST-fusion protein is insoluble. Michael DiDonato
A effective method for elimination of DNA and RNA residues Dnacleaner
(none) Alex Dobrovic
sense and antisense strands Alex Dobrovic
aluI digestion of PCR products Alex Dobrovic
Effect of TE on ligations? Andrew Doherty
tet- free serum Andrew Doherty
Nde I restriction enzyme Andrew Doherty
GFP antibodies? Andrew Doherty
Kinases in HEK293 cells Andrew Doherty
Flumoxing ligation problem Andrew Doherty
Blast, sequence alignment program Michael Douglas
Why does in situ work with sense probes, too? Julian Dow
Genebridge 4/chromosomal localisation. Dr Donald Dunbar
Genetics Position Douglas Easton
DNA probes for use with prokaryote DNA in Southerns Douglas Easton
DNA probes for use with prokaryote DNA in Southerns Douglas Easton
(no subject) Ed
Transposon mutagenesis Ed
Anomalous PCR product Rubin Eitan
Blot with TEFLON membrane, references? Armin Engels
Blot with TEFLON membrane, references? Armin Engels
Bac-to-Bac System Hernan Espinoza
How to wash glassware for using with mRNA experiment Heather Etchevers
in situ hybridization Heather Etchevers
Help Culture of QT-6 cells Heather Etchevers
Bailey's Irish Cream Heather Etchevers
Concanavalin A immobilization Marco Euler
Rescuing plasmid from dead E.coli stock. Frank O. Fackelmayer
NorthWestern protocol Frank O. Fackelmayer
Removing the lipid from the protein sample Frank O. Fackelmayer
Trout Liver cDNA library Patrick HJ Falckh
Hirt extract purification?? Karl Fischer
blunt ligation Karl Fischer
E. coli CJ236 Karl Fischer
Where is anti pan-myc? OM-11-902 Tim Fitzmaurice
where to get pipetman parts Brian A. Flannigan
ultracentrifuge problems Lesleigh Force
studies on aluminum? Heggie Forest
N blot Tama C. Fox
How to measure total protein conc. of cultured-cell lysate? Richard Van Frank
search for DNase I sensitivity assay protocol Olivier Friard
in vitro translation problems Will Fuller
PGal concentration André Gallant
Clonetech André Gallant
Happy New Year André Gallant
Biotin/streptavidin/blocking Mike Garbutt
"clearing" cell culture inserts Mike Garbutt
Nde I restriction enzyme Mike Gardner
cycle sequencing Els Van Geldre
YPD medium and Yeast growth Mohammad Ghahremani
Two hybrid library from Jurkat or HEK293 cells? Mohammad Ghahremani
Probe Assistant Macintosh Software Jason Giles
REF52 Ole Gjoerup
DNA extraction from polysaccharide-laden plant tissue Michelle Gleeson
Licor and phage clones Michelle Gleeson
Problem Blotting of Pulsed Field Gels Michelle Gleeson
?? Kinetics Software - Post David Goldenberg
Blast, sequence alignment program Josh Goldstein
cycle sequencing Marc A. Goldstein
Thrombin Keith Gough
Protease Inhibitors Keith Gough
BstE II, a "good" enzyme ?? Roney Graf
>100% 2nd-strand cDNA ("snapback")? Jim Graham
Need sequence reformatting program for DNASIS David Grant
Cocktail sticks and toothpicks supplier Richard P Grant
Microwave sterilization Richard P Grant
Linear PCR Keith Grimaldi
Ann: SeqSearch v1.0 for Windows, DNA analyis package Niels Groot
Q: using washing powder proteases in molecular biology Mike Gruidl
FK506 calcineurin inhibitor Toumy Guettouche
xenopus egg extract Toumy Guettouche
PCR with Pwo DNA polymerase from Boehringer Toumy Guettouche
oligodt-cellulose Szabadkai Gyorgy
DNA extraction from polysaccharide-laden plant tissue Robert Hamilton
Clonetech Robert Hamilton
6-TG/HPRT Sabine Hanelt
Help on PCR cloning Thomas Hansner
quick hybidizations Heather Hanson
(none) Misako Haraguchi
subcloning Misako Haraguchi
test P.E. Hariharan
barley centimorgan size Neil Harris
DNA Separation Moira Harrison
isocaproaldehyde T. Harter
Addition of 5' phosphate to Riboside Greg Hawkins
unsubscribe J.P. Hays
Nde I restriction enzyme Jared Head
Micronuclei Staining Jared Head
Bailey's Irish Cream Paul N Hengen
Freeze-thawing of primers Paul N Hengen
Cocktail sticks and toothpicks supplier Paul N Hengen
Cocktail sticks and toothpicks supplier Paul N Hengen
Pointer to FAQ list Paul N Hengen
CuraGen Corp Information Paul N Hengen
Desalination Paul N Hengen
Microwave sterilization Paul N Hengen
Cocktail sticks and toothpicks supplier Paul N Hengen
PCR primer design (Rules Of Thumb) Paul N Hengen
Phage display vector request. Paul N Hengen
PCR with Pwo DNA polymerase from Boehringer Paul N Hengen
Microwave sterilization Paul N Hengen
Microwave sterilization Paul N Hengen
RNAse showing up in gel after plasmid miniprep Paul N Hengen
(no subject) Paul N Hengen
Transposon mutagenesis Paul N Hengen
Quality Sticks David Hills
What factors effect protein migration on SDS-PAGE David Hills
Microwave sterilization David Hills
Microwave sterilization David Hills
Cocktail sticks and toothpicks supplier David Hills
DMSO vs. raising annealing temp. in PCR Timo Hiltunen
Silver Staining of DNA Robert Hindges
Microwave sterilization Susan Jane Hogarth
mRNA purification Mike Holbrook
30 Second Ice Cream (Was Re: Bailey's Irish Cream) Louis Hom
Phenol extraction problem/phases not separating Jan Hoolwerf
RNAse showing up in gel after plasmid miniprep Ken Howe
HUVEC Cell freeze & thaw Susan Hubchak
VIRUS WARNING Fred Hughson
DYNAL BEADS Gene Huh
cDNA library optimization Benoît Hébert
test JUAN
Sorry! Simple HRP question Scott Jacoves
Insertional inactivation of genes Farid Jalali
How to measure total protein conc. of cultured-cell lysate? Glenn Jenkins
in-gel kinase assay Glenn Jenkins
for Shou Jiang
Weak Genebank Searches? Jim
RNase H Jillian Johnston
Nde I restriction enzyme Zophonias O. Jonsson
Microwave sterilization (the insect angle!!) Zophonias O. Jonsson
NspB II source? Zophonias O. Jonsson
RNAse showing up in gel after plasmid miniprep Zophonias O. Jonsson
operon searching Ronald Koder Jr
SDS-PAGE SAMPLES KAREN
Reverse Northern KHUANG at CHEMVX.TAMU.EDU
What factors effect protein migration on SDS-PAGE KHUANG at CHEMVX.TAMU.EDU
E. coli CJ236 KHUANG at CHEMVX.TAMU.EDU
Library screening KHUANG at CHEMVX.TAMU.EDU
SSCP not single stranded. D. KIM
Silver Staining of DNA D. KIM
Neurofilament anitbody for ES cells MUN KIN
<no subject> Andrea KREMPLER
salmon sperm DNA Fredrik Kamme
PRC product info Praveen K. Karanth
YPD medium and Yeast growth Maggie Kasten
PCR with Pwo DNA polymerase from Boehringer Don J. Katcoff
PCR with Pwo DNA polymerase from Boehringer Don J. Katcoff
PCR with Pwo DNA polymerase from Boehringer Don J. Katcoff
PCR with Pwo DNA polymerase from Boehringer Don J. Katcoff
phage display Don J. Katcoff
RNAse showing up in gel after plasmid miniprep Jill Kellogg
PhD Position available Gabriele Kerber
RNase removal by phenol-chloroform ? Deepak Khandka
Removing the lipid from the protein sample Dooyeon Kim
densitometry software? Hanbok Kim
papers on the effects of glycerol ? Svend Kjaer
"GATA" probe Andrea Klabouchova
A260/A280 ratios of oligonucleotide Dima Klenchin
EGTA buffer Dima Klenchin
how to remove SDS from RNA sample? Dima Klenchin
alpha-globin Ryszard Kole
Antibiotics for tissue culture John Korte
What makes gels smile? Alexander Kraev
HELP! TBS for GST? Cornelius Krasel
Biotin/streptavidin/blocking Gary Krause
-80 freezer reccomendations Candace Krepel
Protein sticking to glass.. Rajesh Krishnamurthy
Actinomycin D Georg Kroeger
ultracentrifuge problems Georg Kroeger
I'm sorry Georg Kroeger
How long will labelled oligos last? Jerry Kropp
Blood Storage for DNA Extraction Jerry Kropp
Suggestions for vacuum pump Jerry Kropp
How long will labelled oligos last? Jerry Kropp
DMSO vs. raising annealing temp. in PCR Jerry Kropp
Faculty Research Assistant/Plant Molecular Geneticist Konstantin Krutovskii
Help Contacting UVP Inc Gary Kucera
quick hybidizations Gary Kucera
GST-fusion protein is insoluble. Alexander N. Kukushkin
salmon sperm DNA Alexander N. Kukushkin
Cloning Double-Standed Oligos Alexander N. Kukushkin
Maximizer from Clontech Lab. Alexander N. Kukushkin
Library Screening Rajesh Kumar
Role of SDS in hybridization Rajesh Kumar
How to do NATIVE PAGE? Rico Laage
Mutagenesis Kits Payne Lab
Riboprobes Clore Laboratory
Riboprobes Clore Laboratory
PCR tubes quad wells/384 wells John Ladasky
What makes gels smile? John Ladasky
What makes gels smile? John Ladasky
cloning problems John Ladasky
Plant Prep for DNA Extraction Michael Lassner
PC12 Stable transfectant Lee, Hyoung-gon
rice DNA Min-Ho Lee
for Min-Ho Lee
PC12 Stable transfectant Min-Ho Lee
Lambda DNA extraction protocol Lee, Sun
Who sells lactacystin? Brian Hollander and Susan Lenk
AFLP kits ? Estelle Lerceteau
purification of His-tagged proteins Wulf Dirk Leuschner
Hirt extract purification?? Victor Levenson
cDNA library Victor Levenson
Invitrogen's ecdysone-inducible system Victor Levenson
Help on Agarose! Cindy Lin
shareware enzyme kinetics ? Otso Lindy
EGTA buffer Francesco Lipari
Help Culture of QT-6 cells Jan Liphardt
Help Culture of QT-6 cells Jan Liphardt
Invitrogen Electroporator II Yichin Liu
rice DNA Muhammad A. Lodhi
Bp-exchange by PCR Klaus Lun
PCR with Pwo DNA polymerase from Boehringer Klaus Lun
DDRT-PCR Paul C. Grimm MD
Difficulties Obtaining Genomic DNA MELANSOND
Rna gel MELANSOND
gel smear without stop MELANSOND
Removal of PCR DNA contamination MELANSOND
Blot with TEFLON membrane, references? DR. DING MING
GFP antibodies? DR. DING MING
unsubscribe FABIAN MOEBIUS
Folding simulation Maarten
gcg Clinton MacDonald
How long will labelled oligos last? Dr. Michael T. MacDonell
Suggestions for vacuum pump Dr. Michael T. MacDonell
DNA bending John MacDougall
SSCP not single stranded. John MacFarlane
Freeze-thawing of primers David MacHugh
Rescuing plasmid from dead E.coli stock. Emma Macfarlane
phospholipid separation method Neal Machtiger
Phenol extraction problem/phases not separating Deborah Maguire
Actinomycin D Ulrich Maier
DNA yield from plant tissue. Shehrebanoo Malik
Measure pH, ions, O2 in your smallest samples Steve Malin
FREE pH measurement booklet George Mallory
Plasmid DNA from gels Michael Mangan
(no subject) Michael Mangan
Hybridization conditions Mozart de Azevedo Marins
Biotin/streptavidin/blocking Dr. David Martin
SILVER STAINING Dr. David Martin
DNA yield from plant tissue. J. Pat Martinez
pBluescript v. pBluescriptII ?? J. Pat Martinez
Problem Blotting of Pulsed Field Gels J. Pat Martinez
salmon sperm DNA J. Pat Martinez
Volunteers Wanted Marty at mk.ibek.com
salmon sperm DNA Saroj Priyantha Mathupala
Current protocols in molecular biology Sally McCormick
Blast, sequence alignment program Sally McCormick
agrobacterium cosmid library Denise Melanson
degenerate oligos: I or everything? Denise Melanson
suicide plasmid Pascal Mertens
Phage display vector request. Pascal Mertens
Freeze-thawing of primers Joe Miano
Reprobing whole mounts? Joe Miano
mycoplasma Michael R. Freeman, Ph.D.
Cocktail sticks and toothpicks supplier David Micklem
blunt ligation David Micklem
Cocktail sticks and toothpicks supplier David Micklem
DMSO vs. raising annealing temp. in PCR Michael Morales
Removal of PCR DNA contamination John Morrow
What is the typical concentration of a DNA polynucleotide in mols/L after PCR amplification? Ludvig Mortberg
Storing RNA Thibaud Le Mouel
2 cell lines needed ! Heéeéeélp ! Bernard Murray
tet- free serum Bernard Murray
How to measure total protein conc. of cultured-cell lysate? Bernard Murray
Microwave sterilization Bernard Murray
RNAse showing up in gel after plasmid miniprep Bernard Murray
Neomycin resistance Bernard Murray
How to discriminate male and female? Bernard Murray
salmon sperm DNA Bernard Murray
Removal of E.coli non-specific binding Bernard Murray
suicide plasmid WL NG
[Q] detecting a mutation site on a BAC clone Hong- Gil Nam
HUVEC Cell freeze & thaw Tsunehisa Namba
ultracentrifuge problems Kevin Nawotka
Need sequence reformatting program for DNASIS David Neece
Where is anti pan-myc? OM-11-902 Chris Nelson
Bailey's Irish Cream Chia Jin Ngee
Indian Ink supplier Chia Jin Ngee
Clonetech Rick Nicholson
Schneider cells anyone? Soeren Jensby Nielsen
-80 freezer reccomendations Alan Nighorn
Current protocols in molecular biology Gerd Nilsen
DYNAL BEADS Gerd Nilsen
sence strand and antisence strand Gerd Nilsen
Questions on the RNA extraction Alexandre Noël
Cocktail sticks and toothpicks supplier Bob Obar
Silver Staining of DNA Bob Obar
Transfer of large proteins (>500 kD) to nitrocellulose. How? Bob Obar
Nde I restriction enzyme Martin Offterdinger
Nde I restriction enzyme Martin Offterdinger
How to measure total protein conc. of cultured-cell lysate? Martin Offterdinger
BstE II, a "good" enzyme ?? Martin Offterdinger
PCR with Pwo DNA polymerase from Boehringer Martin Offterdinger
Automatic sequence analyzer??? Takbum Ohn
FK506 calcineurin inhibitor Van Wuytswinkel Olivier
Ni(II)SO4 The Kindly One
Double stranded oligonucleotide's denaturation under CH3CN... Sergey V. Orlov
Virtual PFGE Software? Nick Owen
removing e.coli non-specific activity from antisera DAVID PULFORD
Removal of E.coli non-specific binding DAVID PULFORD
Protease Inhibitor Cocktails Richard C. Padgett
Help with random subcloning of YAC Veera Padmanabhan
Gel purification of YAC DNA Veera Padmanabhan
Bioinformatics Gurcharan Pahwa
Tn10 Sequence Elise Palmer
Lambda DNA extraction protocol Elise Palmer
Storm Reliability Rodgers Palmer
test Andre Charles Paquin
HCMV plque assay Chung-Gyu Park
Nitrocellulose membrane and UV cross-linking. Yoonsuk Park
On again off again PCR Tara Paton
Gel shifts: run them fast or slow? John Pawlowski
DNA extraction from polysaccharide-laden plant tissue Steve Pechous
Phenol extraction problem/phases not separating Pete
A260/A280 ratios of oligonucleotide Pete
Bailey's Irish Cream Jennifer Philhower
Plant rRNAs Andy Phillips
Ligation tips SM Pickering-Brown
Ligation tips? I'm ligating double stranded adaptors (with various sticky ends) to Dynal bead immobilised DNA (with the appropriate sticky ends!) and I was wondering if anyone has got any 'top tips' for for increasing the eff SM Pickering-Brown
Plant rRNAs Markus Piotrowski
lambda charon -source Venkat Pisupati
in vitro translation problems Mircea Podar
run-off translation in vitro? Scott Vande Pol
RNase removal by phenol-chloroform ? Bo Pontoppidan
Good luminometers Phil Potter
[Q] detecting a mutation site on a BAC clone Michael J. Prigge
plasmid vector with lambdaGEM11-like SfiI sites? Michael J. Prigge
Microwave sterilization Michael J. Prigge
pUC21 sequence? (or..."pSK"= "pBluescript SK") Mike Prigge
Rules of thumb for messing with vector's LacZ' coding seq? Mike Prigge
pBluescript v. pBluescriptII ?? Mike Prigge
Microwave sterilization Mike Prigge
Ligation problems... SARAH LOUISE RAWLINGS
Questions on the RNA extraction REGCHAK at USTHK.UST.HK
total [RNA] in Arabidopsis REGCHAK at USTHK.UST.HK
DNA Prep for PCR from bacterial spores ? RManns7
"clearing" cell culture inserts RManns7
Help Contacting UVP Inc Karthi Ramachandran
Software for counting colonies? Karthi Ramachandran
Help on PCR cloning Ramanujam Raman
Separation of two proteins with similar properties Marit Valeur Ramstad
Baileys Irish Cream Colin Rasmussen
Plant DNA Extraction Soren K. Rasmussen
Purifying DNA from gel slices - Summary Alison Reavley
?? Kinetics Software - Post Achim Recktenwald
Smeared Bands in PCR Reid, Thomas M.
NorthWestern protocol Mary P. Remington
Beatrice Macchi email address Mary P. Remington
Transfer of large proteins (>500 kD) to nitrocellulose. How? Gunther Rezniczek
(no subject) Riaga
Primer design software John H Richburg
30 Second Ice Cream (Was Re: Bailey's Irish Cream) Robert W. St. G. Fisher, IV
rifampicine Marilyn Roberts
Sequencing an A-T region Tom Robinson
Amplyfing cDNA libraries Robmit
Cloning Double-Standed Oligos Lourdes Rodgers
RNase H Lourdes Rodgers
gcg MIcha Ron
native vs denaturing gel for RPA Mike Rott
Plasmid contamination from pipettors Rover
nick Translation Krish Roy
RNase removal by phenol-chloroform ? Hiranya Roychowdhury
Lambda DNA extraction protocol Hiranya Roychowdhury
Nitrocellulose membrane and UV cross-linking. Hiranya Roychowdhury
Rescuing plasmid from dead E.coli stock. Hiranya Roychowdhury
blunt ligation Hiranya Roychowdhury
denaturing DNA in 0.12Mpb Hiranya Roychowdhury
Help on PCR cloning Hiranya Roychowdhury
Anomalous PCR product Hiranya Roychowdhury
How do you dissolve NBT and BCIP? Hiranya Roychowdhury
Protein sticking to glass.. Hiranya Roychowdhury
RNAse showing up in gel after plasmid miniprep Hiranya Roychowdhury
pBluescript v. pBluescriptII ?? George Rutherford
BRL ZipLox George Rutherford
Ligation problems... George Rutherford
blunt end !!! George Rutherford
RNAse showing up in gel after plasmid miniprep George Rutherford
trimming polyA tails SCLAB
Pfu polymerase PETER S. SILVERSTEIN
0.75 mm spacers and comb Juergen Sasse
Gel shifts: run them fast or slow? Heinz-Juergen Schaefers
Separation of two proteins with similar properties Jon-Sverre Schanche
tet- free serum Wolfgang Schechinger
inclusion bodies (IBs), purification of Wolfgang Schechinger
Your GOD Loves You - YES YOU! Wolfgang Schechinger
DNA software Wolfgang Schechinger
Protein sticking to glass.. Wolfgang Schechinger
blunt end !!! Wolfgang Schechinger
Protease Inhibitors Wolfgang Schechinger
phospholipid separation method Wolfgang Schechinger
Q: using washing powder proteases in molecular biology Wolfgang Schechinger
inclusion bodies, purification of Karin Schott
Nitrocellulose membrane and UV cross-linking. Kathleen Schroeder
solid phase cDNA library Bernhard Schu
anti hamster GR antibody Phillip E. Schwartz
Cloning Double-Standed Oligos Jeff "Newt" Sekelsky
expression/fusion vectors Bret Sellman
Cloning Double-Standed Oligos Jurgen Seppen
Rnase removal with phenol:chloroform Shuiliang Shi
Phage display vector request. Igor Sidorenkov
FK506 calcineurin inhibitor S. Silverman
PCR tubes Rob Skilton
VP16 monoclonal Mark Slack
Cloning PCR products Ken Soderstrom
Telomerase quantification Jackie Song
Telomerase quantification Jackie Song
Telomerase quantification Jackie Song
Microplate Conference MIP-TEC 97 Keld Sorensen
Glutathione Magnetic Beads Keld Sorensen
removing e.coli non-specific activity from antisera Keld Sorensen
trimming polyA tails Augustine Souza
Riboprobes, Northerns, and high background Mart Speek
Help with Lysis Buffer 4 adherent cells please ! Mart Speek
composition of the ER lumen Sebastian Springer
Q: Flow cytometry with anti-flag? Reuven Stein
Indian Ink supplier Jørgen Stenvik
Histones/poly(A) question Steven Sullivan
T/A PCR cloning Steven Sullivan
Gel Documentation: problems with UVP Dennis Summerbell
EtBr Gel documentation systems-UVP Dennis Summerbell
Protein sticking to glass.. Dennis Summerbell
2 cell lines needed ! Heéeéeélp ! Clemens Suter
Bp-exchange by PCR Vladimir Svetlov
Help on PCR cloning Vladimir Svetlov
Mutagenesis Kits Vladimir Svetlov
Purifying DNA from gel slices - Summary Vladimir Svetlov
dna degradation Vladimir Svetlov
PCR primer design (Rules Of Thumb) Vladimir Svetlov
Nitrocellulose membrane and UV cross-linking. Michal Svoboda
Histones/poly(A) question Marci Swede
-80 freezer reccomendations H. Allen Sylvester
How do you dissolve NBT and BCIP? H. Allen Sylvester
UNSTABLE TRIPLETS, MICROSATELLITES, Cambridge Symposia
Fourth Annual HUMAN GENOME PROJECT: Commercial Implications Meeting Cambridge Symposia
GENETIC SCREENING AND DIAGNOSIS OF HUMAN DISEASES Cambridge Symposia
Functional Genomics:From Genes To Drugs Cambridge Symposia
Fumehoods fans T.Downing
COOL lab protocol web site ?? YangLin from Taiwan
how to remove SDS from RNA sample? Seng-Lai Tan
how to remove SDS from RNA samples Seng-Lai Tan
Chromosomal DNA from Actinomyces Tapani.Reinikainen at cultor.fi
Protein sequencing ??!! Ron Tate
How to do NATIVE PAGE? Ron Tate
Linear PCR Chromosome Terror
Histones/poly(A) question Tom Thatcher
quick hybidizations Michael W. Thompson
Plasmid contamination from pipettors Michael W. Thompson
Library Screening Michael W. Thompson
Lamda Sequencing... Ulrich Tillmann
DNA Extraction Protocol Tim
Rules of thumb for messing with vector's LacZ' coding seq? Clive Tregaskes
Amplyfing cDNA libraries Clive Tregaskes
Help Jonathan Tugwood
Seeking DOS/WIN reader for UNIX "mail" files Bradley Turner
expression/fusion vectors Anton Tutter
Heat inactivation of the phosphor diester bond in DNA Anton Tutter
Transfer of large proteins (>500 kD) to nitrocellulose. How? Anton Tutter
Protocol- hybridization with oligonucleotides Przemko Tylzanowski
Folding simulation Panapat Uawithya
Plasmid Isolation from yeast The User
scfv, MoAb, cell targeting. looking for... Antoine VEKRIS
suicide plasmid Edgar Valencia
sence strand and antisence strand Edgar Valencia
mathematical simulation in immunology Oleg S. Vasilyev
a question on PCR of bacterial colonies nathalie Viguerie
cloning problems Dr A.H.M. Van Vliet
Q: using washing powder proteases in molecular biology Sam Vonarx
dna degradation Karl Voss
anti hamster GR antibody WENCENLAUS
0.75 mm spacers and comb WSchick at aol.com
PCR tubes quad wells/384 wells WSchick at aol.com
PCR loading/384 wells/864 wells/?? WSchick at aol.com
DNA software (Hybridization) WSchick at aol.com
Biotechniques article WSchick at aol.com
Beatrice Macchi email address (whowhere?) WSchick at aol.com
Primer design software WSchick at aol.com
Cocktail sticks and toothpicks supplier Andrew Wallace
"reverse northern" problems Ed Wang
Storing RNA Ed Wang
Reverse Northern Ed Wang
Linear PCR Ed Wang
cDNA library Ed Wang
Blast, sequence alignment program John Watson
CAT assay kits John Webster
DNA extraction from polysaccharide-laden plant tissue Chi-kuang Wen
cDNA library amplification Chi-kuang Wen
Library Screening Chi-kuang Wen
Cocktail sticks and toothpicks supplier Chi-kuang Wen
6-TG/HPRT Joseph Leo Wiemels
elastase cDNA Malgorzata Wilczynska
PCR Problem Jenny Williams
Weak Genebank Searches? Tori D. Williams
pCMV B-gal Tori D. Williams
plastic supplies Wilson_Lab
Help Bruce Windoffer
What makes gels smile? Neil Winegarden
How to measure total protein conc. of cultured-cell lysate? David Winterbourne
Cocktail sticks and toothpicks supplier David Winterbourne
trimming polyA tails Graeme Wistow
REQ: PCR primers - help wanted Harry Witchel
K+ channel expression in CHO cells only lasts 15 minutes Harry Witchel
Cocktail sticks and toothpicks supplier Harry Witchel
Eucaryotic protein translation, ribosomes and ribosomal proteins-looking for references Stephan Witte
Plant rRNAs Hongyu Yang
Amiloride stocks Ian A. York
Amplyfing cDNA libraries Ian A. York
blunt end !!! Ian A. York
Pfu polymerase Ian A. York
Invitrogen's ecdysone-inducible system Ian A. York
Removal of E.coli non-specific binding Ian A. York
Qualtification of EtBr-stained DNA bands. Zhongtang Yu
Role of SDS in hybridization Man Z
How can I cofirm differential expression ? Man Z
cell lines Bob Zahorchak
How do I measure H2 in solution ? Hayssam Zakaria
anti hamster GR antibody Matthias Zeiner
capped RNA isolation - help wanted Shuang Zhang
Why don't my plasmid freeze in freezer? Marieke R. Koedood Zhao
Gel shifts: run them fast or slow? Marieke R. Koedood Zhao
Plasmid contamination from pipettors Marieke R. Koedood Zhao
salmon sperm DNA Marieke R. Koedood Zhao
A260/A280 ratios of oligonucleotide Zhiyong Zhao
Ligation problems... Zhiyong Zhao
about calcitonin gene Liu Zhen
sence strand and antisence strand Liu Zhen
quick hybidizations anat-hd
How to get rid of primer anat-hd
RNase removal by phenol-chloroform ? eric anderson
-80 freezer reccomendations eric anderson
mRNA purification eric anderson
"reverse northern" problems eric anderson
plastic supplies eric anderson
Microwave sterilization eric anderson
Questions on the RNA extraction eric anderson
where to get pipetman parts eric anderson
How can I cofirm differential expression ? eric anderson
Suggestions for vacuum pump arkcon at ibm.net
Biotin/streptavidin/blocking brett
What makes gels smile? brett
How to remove RNase from protein extract? brett
how to remove SDS from RNA sample? brett
Help Contacting UVP Inc bruce
Gene Quantification 2nd Annual Conference announcement mary chitty
Plant rRNAs chriso at dfrc.wisc.edu
salmon sperm DNA darren cikaluk
contents of Chinese Journal of Veterinary Science cjvs at PUBLIC.CC.JL.CN
contents of chinese journal of veterinary science cjvs at PUBLIC.CC.JL.CN
SDS-PAGE SAMPLES davesmith at bioch.tamu.edu
protein expression system doumas
blunt end !!! edd at okstate.edu
"reverse northern" problems eheinecke at STUDENTS.WISC.EDU
L8 myoblast cell line etud.bta at istab.u-bordeaux.fr
DNA software doug feinstein
TH-Assay frank.narz at rz.ruhr-uni-bochum.de
What factors effect protein migration on SDS-PAGE gc
SSCP band smearing geert at lmb1.rug.ac.be
glycoprotein,quantitation li chun hai
exon mapping hdong at iastate.edu
inclusion bodies, purification of hdz at aol.com
Composite gels for SSCP Administrador de iihema.fmed.uba.ar
What makes gels smile? Administrador de iihema.fmed.uba.ar
How long will labelled oligos last? Administrador de iihema.fmed.uba.ar
in-gel kinase assay fan jiang
Mitochondrial DNA cloning jmorrow at bio.usyd.edu.au
PLEASE HELP!!! porankiewicz joanna
HeLa pellet supplier? jstrassw at OPAL.TUFTS.EDU
Protein sequencing ??!! jstrassw at OPAL.TUFTS.EDU
Vacuoles in HeLa cells? jstrassw at OPAL.TUFTS.EDU
degenerate oligos: I or everything? jstrassw at OPAL.TUFTS.EDU
plastic film cassettes karynvan at bu.edu
How can I cofirm differential expression ? khsw124
Storm Reliability kramerse at wmvx.lvs.dupont.com
Clonetech kramerse at wmvx.lvs.dupont.com
parathyroid physiological levels chandira kumar
Biotechniques article charles lawrie
GST-fusion protein is insoluble. lina at PUBMS.PKU.EDU.CN
How to dissolve NBT and BCIP ---> not in DMFA ! lina at PUBMS.PKU.EDU.CN
Nde I restriction enzyme lj1 at mrc-lmb.cam.ac.uk
Phenol extraction problem/phases not separating mac-biocomputing1user
Protein sequencing ??!! mac-biocomputing1user
Methylation of Plasmid !!! mac-biocomputing2user
PCR Problem marko
Can blotted enzymes retain there activity on nitrocellulose? marko
aluI digestion medbh at biovax.leeds.ac.uk
aluI digestion problem medbh at biovax.leeds.ac.uk
the three and prime ends of proteins mlf at engr.uark.edu
dna degradation mobio at electriciti.com
Heat inactivation of the phosphor diester bond in DNA mobio at electriciti.com
What makes gels smile? mullenlynne at .jsei.ucla.edu
How long will labelled oligos last? mullenlynne at jsei.ucla.edu
myosin antibodies naho.yamazaki at seh.ox.ac.uk
RNA STAINING natldiagnos at mindspring.com
Qualtification of EtBr-stained DNA bands. natldiagnos at mindspring.com
how to remove SDS from RNA sample? nestgrp at earthlink.net
Help nhwu at PUBLIC3.BTA.NET.CN
A260/A280 ratios of oligonucleotide nhwu at PUBLIC3.BTA.NET.CN
How to remove RNase from protein extract? nhwu at PUBLIC3.BTA.NET.CN
How to wash glassware for using with mRNA experiment nhwu at PUBLIC3.BTA.NET.CN
RNase removal by phenol-chloroform ? nierlich at ucla.edu
Using heterogenous genomic DNA fragments as probes in Southern Blots patricia ohana
Chromosome Puls-field oliver.kudlacek at univie.ac.at
RT PCR woes... parakeet
a question on PCR of bacterial colonies parakeet
degenerate oligos: I or everything? parakeet
SSCP not single stranded. pdxrmb at vaxb.ccc.nottingham.ac.uk
DNA Prep for PCR from bacterial spores ? raja at itsa.ucsf.edu
VIRUS WARNING bruce w. ritchings
PCR tubes quad wells/384 wells rmanns7 at aol.com
Poste de Professeur à l'IUT de Quimper robreau at iutquimp.univ-brest.fr
nih image-help- rubeniac at HUEMUL.FFYB.UBA.AR
HELP ON HYBRIDIZATION sahingur at acsu.buffalo.edu
Good luminometers ole morten seternes
removing e.coli non-specific activity from antisera sharmaa at acsu.buffalo.edu
how dangerous is ethidium bromide slavemaster
Phenol extraction problem/phases not separating slavemaster
BMP or GDF activity assay slavemaster
Freeze-thawing of primers slavemaster
inclusion bodies, purification of slavemaster
inclusion bodies (IBs), purification of slavemaster
2 cell lines needed ! Heéeéeélp ! slavemaster
nt2 / hnt2 cell question slavemaster
BCP supplier? cynthia stryker
cloning problems thees at mzdmza.zdv.uni-mainz.de
What makes gels smile? Matt thomas
precipitation of RNA transcripts train4train
Protocol- hybridization with oligonucleotides txpljfg at uabcvsr.seas.ucla.edu
DANGER:where is metylmercuric hydroxide? unglee
HELP:BBWV cDNA library unglee
HELP:BBWV cDNA library unglee
Lysis of bacteria in maxi prep. vilimf01 at mcrcr6.med.nyu.edu
Riboprobes vilimf01 at mcrcr6.med.nyu.edu
Bp-exchange by PCR vilimf01 at mcrcr6.med.nyu.edu
PCR with Pwo DNA polymerase from Boehringer vilimf01 at mcrcr6.med.nyu.edu
Smeared Bands in PCR vilimf01 at mcrcr6.med.nyu.edu
How do you dissolve NBT and BCIP? xinguo at acsu.buffalo.edu
Genomic DNA from JAX yockey at dcsmserver.med.sc.edu
Antibodies toVP16 ziffe01 at mcrcr6.med.nyu.edu
Storm Reliability zinc
phospholipid separation method zinchenk at com2com.ru
blunt end !!! §Ú¬O·R·ÓÃè¤lªºmutant!
bacteriophage f1 §Ú¬O·R·ÓÃè¤lªºmutant!
Reamplification problem of the eluted PCR amplicons from PAGE gel »ýÃæÇÐ±³½Ç ±³¼ö È«¿ëÇ

Last message date: Tue Dec 31 19:39:21 EST 1996
Archived on: Sun Mar 6 21:16:25 EST 2005

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