Immunoprecipitation using proteinA agarose

kim myung jong pgs at vision.postech.ac.kr
Fri Feb 2 01:53:48 EST 1996


Hi! everyone.
I am graduate student in south korea.
I want to know tip that can reduce non-specific association of cell 
extract to protein A agarose in immunoprecipitation experiment.
I routinly add antibody solution to pre-cleared mammalian cell 
extract and then add protein A agarose.
In my opinion, nonspecific binding is due to washing buffer.
My washing buffer contain 1% Triton X-100 and 180mM NaCl.
Please, give me solution.
Thanks in advance.
My email address is
pgs at vision.postech.ac.kr
Myung Jong Kim



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