DNA from whole blood

Sabine Hanelt sabine.hanelt at medizin.uni-ulm.de
Thu Feb 1 02:25:39 EST 1996


Hey all there,
it seems as if only few people are working with whole blood. Because I only 
got few protocols for isolating nuclei from blood. My problem is that I need 
all blood cells, not only the lymphocytes. Therefore my question:
Any good protocol for nuclei isolation and do I need a special washing buffer 
(or can I use the lysisbuffer as washing buffer, or can I use PBS?)
Thanks for your help
Sabine 

Sabine Hanelt
Abt. Medizinische Genetik
Uni Klinik Ulm
D-89069 Ulm
Phone: +49-731-502-3432
Fax: +49-731-502-3438



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