"Marianne Leverone ", BIO
leverone at CHUMA.CAS.USF.EDU
Thu Feb 1 10:39:30 EST 1996
Dear D.K.: N. meningitidis is pretty darn picky. You may want to get a
hold of a book that describes this bug. It requires 5% CO2 for growth so
you will have to use a 5%CO2 incubator or a candle jar. I don't know how
weel it grows in a canle jar however (a big mayonaisse jar with petri
plates with a short candle on top; light the candle, close the lid
tightly -air tight; the candle burns out- oxygen has been reduced).
ATCC says N. meningitidis grows on chocolate agar, a rich medium (GC
agar base) with dried hemoglobin and IsovitaleX (BBL 11876). You can
also use GC base with blood cells; heat the cells to ~80C until the
medium turns to a chocolate color and temper immediately to 45-50C and
pour plates (or just buy some pre-poured). This species may only survive
about 2 days before you will have to restreak. I don't know about your
other questions. Maybe you better talk to someone who works with this
organism. Marianne Leverone, USF, Tampa
On Wed, 31 Jan 1996, David Karaolis wrote:
> Although my main work is on enterics, I am about to start some work ("in
> my spare time") on Neisseria meningitidis.
> Could someone please answer the following:
> 1) What is the best storage medium?
> 2) Do they grow well in LB broth/LB agar?
> 3) How long do they stay viable on agar at RT and at 4oC ?
> 4) Do they grow in Eagles Minimal Essential Medium (EMEM)?
> 5) Do they easily transform/electroporate with E. coli plasmids?
> Thanks for any help.
> David K.R. Karaolis, Ph.D.
> Center for Vaccine Development
> University of Maryland School of Medicine
> ph: 410-706 2493
> fax: 410-706 6205
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